技术简介
SeekOne® DD单细胞3'转录组测序是寻因生物商业化的首个油包水平台高通量单细胞转录组产品,基于微流控技术原理,利用Barcoded Beads实现单细胞的分离与标记,整体数据水平达到国际领先水平,是揭示细胞异质性的科研利器。
技术简介
优势亮点
极致性价比
高通量单细胞基因表达捕获
项目经验丰富
五万余例样本解离经验,实验成功率高达95%
全国布局响应快
全国化实验室布局,多地保障,24h快速响应
数据解析尽在一指间
SeekSoul online云平台一键开启生信分析
应用范围广
覆盖动植物细胞的分子机制与功能解析研究
技术流程

单细胞解离/提核

油包水逆转录及标记

cDNA扩增建库

上机测序

数据分析和可视化

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技术应用
01
绘制组织全景,解析细胞异质性的基础蓝图
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02
捕捉精细差异,挖掘关键功能细胞的精准定位
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03
揭示致病机制,探索临床诊疗的潜在靶点
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04
解析免疫动态,解锁免疫治疗的核心规律
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05
解析发育轨迹,解码生殖与神经器官的干细胞分化调控机制
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06
精准分型疾病,挖掘临床转化的核心生物标志物
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数据表现
细胞数捕获1W左右,测序100G左右
Species
Sample
Estimated Number of Cells
Median Genes per Cell
Valid Barcodes
Sequencing Saturation
Reads Mapped Confidently to Genome
Fraction Reads in Cells
Total Genes Detected
Species:
Sample: PBMC
Estimated Number of Cells: 11978
Median Genes per Cell: 2609
Valid Barcodes: 91%
Sequencing Saturation: 65%
Reads Mapped Confidently to Genome: 89%
Fraction Reads in Cells: 83%
Total Genes Detected: 27578
Species:
Sample:
Estimated Number of Cells: 10579
Median Genes per Cell: 3077
Valid Barcodes: 93%
Sequencing Saturation: 47%
Reads Mapped Confidently to Genome: 91%
Fraction Reads in Cells: 83%
Total Genes Detected: 28868
Species:
Sample: 肝脏
Estimated Number of Cells: 12275
Median Genes per Cell: 3030
Valid Barcodes: 94%
Sequencing Saturation: 52%
Reads Mapped Confidently to Genome: 86%
Fraction Reads in Cells: 86%
Total Genes Detected: 31474
Species:
Sample: 胶质瘤
Estimated Number of Cells: 12397
Median Genes per Cell: 2794
Valid Barcodes: 92%
Sequencing Saturation: 47%
Reads Mapped Confidently to Genome: 87%
Fraction Reads in Cells: 82%
Total Genes Detected: 30187
Species:
Sample: 胆管癌
Estimated Number of Cells: 10956
Median Genes per Cell: 2375
Valid Barcodes: 91%
Sequencing Saturation: 59%
Reads Mapped Confidently to Genome: 93%
Fraction Reads in Cells: 87%
Total Genes Detected: 29955
Species:
Sample: 皮肤
Estimated Number of Cells: 11858
Median Genes per Cell: 3184
Valid Barcodes: 95%
Sequencing Saturation: 69%
Reads Mapped Confidently to Genome: 90%
Fraction Reads in Cells: 87%
Total Genes Detected: 31748
Species:
Sample:
Estimated Number of Cells: 11972
Median Genes per Cell: 3329
Valid Barcodes: 97%
Sequencing Saturation: 61%
Reads Mapped Confidently to Genome: 88%
Fraction Reads in Cells: 81%
Total Genes Detected: 31607
Species: 小鼠
Sample: 脂肪
Estimated Number of Cells: 12915
Median Genes per Cell: 3137
Valid Barcodes: 92%
Sequencing Saturation: 63%
Reads Mapped Confidently to Genome: 92%
Fraction Reads in Cells: 80%
Total Genes Detected: 25834
Species: 小鼠
Sample:
Estimated Number of Cells: 9618
Median Genes per Cell: 3951
Valid Barcodes: 93%
Sequencing Saturation: 73%
Reads Mapped Confidently to Genome: 89%
Fraction Reads in Cells: 82%
Total Genes Detected: 24193
Species: 小鼠
Sample:
Estimated Number of Cells: 12389
Median Genes per Cell: 3060
Valid Barcodes: 95%
Sequencing Saturation: 68%
Reads Mapped Confidently to Genome: 90%
Fraction Reads in Cells: 81%
Total Genes Detected: 23724
Species: 小鼠
Sample: 乳腺
Estimated Number of Cells: 10467
Median Genes per Cell: 3207
Valid Barcodes: 94%
Sequencing Saturation: 64%
Reads Mapped Confidently to Genome: 88%
Fraction Reads in Cells: 82%
Total Genes Detected: 25004
Species: 小鼠
Sample: 卵巢
Estimated Number of Cells: 12106
Median Genes per Cell: 2426
Valid Barcodes: 93%
Sequencing Saturation: 50%
Reads Mapped Confidently to Genome: 86%
Fraction Reads in Cells: 85%
Total Genes Detected: 23918
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数据集
样本来源:健康人PBMC样品
产品方案:SeekOne® DD单细胞3'转录组
物种:人
类型:PBMC
样本保存:新鲜
上机细胞数:20000
测序平台:真迈生物SURFSeq 5000
RNA测序数据量:100G
样本来源:健康人PBMC样品
产品方案:SeekOne® DD单细胞3'转录组
物种:人
类型:PBMC
样本保存:新鲜
上机细胞数:20000
测序平台:Illumina NovaSeq 6000
RNA测序数据量:100G