技术简介
SeekOne® DD 单细胞5'转录组+表面蛋白测序技术基于微 流控技术原理,同时捕获单细胞内的RNA和结合在细胞表面蛋白的抗体核酸标签信号,实现RNA表达与表面蛋白表达的定性和定量检测。
技术简介
优势亮点
一胞多检
同时检测细胞表面蛋白和转录组信息
通量远超流式分选
单次实现高达千重的表面蛋白检测
细胞功能表征更全面
RNA+蛋白联合表征细胞功能
弥补单细胞转录组dropout
还原真实基因表达状态
技术流程

样本处理

油包水逆转录及标记

转录组cDNA扩增建库&表面蛋白富集建库

上机测序

数据分析和可视化

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技术应用
01
精准细胞类型注释
新型/平见坦胞亚群注释
组胞功能状态表征
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02
富集关注细胞类型
提高CAR检出效率
发现抗原特异性抗体
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03
精准细胞溯源
不惧坦胞量少
真实排除双胞
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数据表现
细胞数捕获1W左右,转录组测序100G左右,表面蛋白测序10G左右
Specles
Sample
Estmated Number Cells
Median Genes per Cell
Sequencing Saturation
Reads Mapped Confidently to Genome
Fraction Reads in Cells
Total Genes Detected
Specles: 小鼠
Sample: 皮下瘤
Estmated Number Cells: 11308
Median Genes per Cell: 2841
Sequencing Saturation: 43.25%
Reads Mapped Confidently to Genome: 89.64%
Fraction Reads in Cells: 87.22%
Total Genes Detected: 25468
Specles: 小鼠
Sample: 肝癌
Estmated Number Cells: 12286
Median Genes per Cell: 2652
Sequencing Saturation: 53.47%
Reads Mapped Confidently to Genome: 91.52%
Fraction Reads in Cells: 89.91%
Total Genes Detected: 24109
Specles:
Sample: 肺癌
Estmated Number Cells: 10171
Median Genes per Cell: 3156
Sequencing Saturation: 44.96%
Reads Mapped Confidently to Genome: 86.99%
Fraction Reads in Cells: 91.56%
Total Genes Detected: 31079
Specles:
Sample: PBMC
Estmated Number Cells: 10732
Median Genes per Cell: 2852
Sequencing Saturation: 55.20%
Reads Mapped Confidently to Genome: 92.42%
Fraction Reads in Cells: 88.38%
Total Genes Detected: 27522
Specles:
Sample: 结直肠癌
Estmated Number Cells: 11209
Median Genes per Cell: 2584
Sequencing Saturation: 47.17%
Reads Mapped Confidently to Genome: 88.51%
Fraction Reads in Cells: 86.24%
Total Genes Detected: 29681
Specles
Sample
Estimated Number of Cells
Fraction Antibody Reads
Fraction Antibody Reads usable
Fraction Unrecognized Antibody
Sequencing Saturation
Specles:小鼠
Specles:皮下瘤
Specles:11308
Specles:95.64%
Specles:52.88%
Specles:4.36%
Specles:68.28%
Specles:小鼠
Specles:肝癌
Specles:12286
Specles:98.13%
Specles:59.25%
Specles:1.87%
Specles:59.64%
Specles:
Specles:肺癌
Specles:10171
Specles:92.51%
Specles:54.37%
Specles:7.49%
Specles:53.95%
Specles:
Specles:PBMC
Specles:10732
Specles:93.67%
Specles:63.94%
Specles:6.33%
Specles:48.00%
Specles:
Specles:结直肠癌
Specles:11209
Specles:95.39%
Specles:61.98%
Specles:4.61%
Specles:55.68%
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